Anti-Brg1 [4E5 ] Ab00905-8.4 from Absolute Antibody

Product Specs
ItemAnti-Brg1 [4E5 ]
CompanyAbsolute Antibody
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAb00905-8.4
Quantity200 µg
ApplicationsIF, IP, WB, IHC
Conjugate/TagUnconjugated, AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
TargetBrg1
IsotypeIgG2b
Clone4E5
HostRat
ImmunogenAnti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting
NCBI Full Gene NameSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Gene AliasesBAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b
ClonalityMonoclonal
ReactivityHu, Ms
Research AreaChromatin; epigenetics
PurityProtein A affinity purified